One of the most important techniques you will learn this semester is how to streak for isolation. As I learned, the streak plate method is a rapid qualitative isolation method. Incubate the plate at … 3. A common method for the isolation of a pure culture from a mixture is by "streaking" plates. Turn plate 90*, streak again using the same wrist motion, repl…. Although there is a number of streaking method but the most common method is quadrant streak method. Streak the mixed culture back and forth the agar in first quad…. In practice, a gradient of cells is established on the surface of an agar plate. The inoculum is streaked over the agar surface to isolate colonies on at least a portion of the plate. Flame the glass spreader over a bunsen burner. Streak the plate with the inoculating loop being careful not to gouge the agar, flame the loop, allow it to cool and finish streaking the plate How to streak a plate from quadrant 1-4 Do not obtain a new culture of bacteria when streaking quadrants 2-4. STREAKING: The streak plate method is a rapid qualitative isolation method. RE Loop is sterilized and a loopful of inoculum is removed from the tube Initial streak is worked in well in one corner of agar plate Appearance of well-streaked plate after incubation shows colonies of Micrococcus luteus on a blood agar plate Subsequent streaks are at angles to the first streak COORD … The streak plate method can be used when starting a Microbiologics product such as a KWIK-STIK™ or when simply sub-culturing a colony from one agar plate to another. It is essentially a dilution technique that involves spreading a loopful of culture over the surface of an agar plate. Step 3 - Streak Plate. Streak Plate method to Purify Single bacteria Performing a Plate Streak I 1: Flame metal inoculating loop, let cool momentarily. Step 2 - Streak Plate. Freshly isolated colonies should be used when performing biochemical, genetic, or antimicrobial susceptibility testing. Streak Plate Method of Isolation Purpose The streak plate technique is the most widely used method of obtaining isolated colonies from a mix of cultures. This is a simple & rapid method. Principle The streak plate technique is essentially a method to dilute the number of organisms, decreasing the density. Streak plate – the original culture is directly diluted across an agar surface using and inoculating loop. It is essentially a dilution technique used for isolation of discrete colonies and requires that the number of organisms in the inoculums be reduced. 2-3: Using sterile technique transfer a loop of bacterial culture or single colony onto loop 4: With one hand remove lid of dish. In essence, the streak plate technique is a type of dilution scheme in which a single colony presumably arises from a single cell. All of these methods dilute or “thin out” a heavy population of bacteria across an agar surface. As you might guess, the purpose of streaking for isolation is to produce isolated colonies of an organism on an agar plate. One very common way to isolate bacterial and other microbes is by employing the streak plate technique. So, the basic objective is to isolate the pure microbial cultures from inoculum. Turn plate 90*, streak again using the same wrist motion, repl…. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of droplets of condensed moisture. Flame loop from base to tip until it turns red hot, let cool,…. This is useful when you need to separate organisms in a mixed culture or when you need to study the colony morphology of an organism. 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